c-Jun Polyclonal Antibody
Catalogue Number: BS-0670R-BSS
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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-C-Jun Polyclonal Antibody (bs-0670R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
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HepG2 cells probed with c-Jun Polyclonal Antibody, unconjugated (bs-0670R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with C-jun Polyclonal Antibody(bs-0670R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Lane 1: Mouse Kidney lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Human Hela cell lysates probed with c-Jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | AP1; AP-1; c-Jun; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human c-Jun |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Additional Text
Gene ID
3725
Gene Name
JUN
Uniprot ID
P05412
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0670R Datasheet
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DELIVERY
we offer free delivery to UK universities and non profit organisations