IL-10 Polyclonal Antibody
Catalogue Number: BS-0698R-BSS
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Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Mouse spleen lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded human colon tissue labeled Anti-IL-10 Polyclonal Antibody, Unconjugated(bs-0698R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Lane 1: Human THP-1 cell lysates; Lane 2: Human THP-1-LPS cell lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CSIF; Il-1; Interleukin-1; Cytokine synthesis inhibitory factor; Il1; il10 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse IL-10 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Additional Text
Gene Name
IL10
Gene ID
16153
Uniprot ID
P18893
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0698R Datasheet
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DELIVERY
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