HSP90 alpha Polyclonal Antibody
Catalogue Number: BS-10100R-BSS
bs-10100R Image
bs-10100R Image
bs-10100R Image
bs-10100R Image
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP90 alpha Polyclonal Antibody, Unconjugated (bs-10100R) at 1:400 overnight at 4°C, DAB staining.
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Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human Molt-4 cell lysates probed with HSP90 alpha Polyclonal Antibody, Unconjugated (bs-10100R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bs-10100R Image
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP90 alpha) polyclonal Antibody, Unconjugated (bs-10100R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human HSP90 alpha |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Additional Text
Gene Name
HSP90AA1
Gene ID
3320
Uniprot ID
P07900
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp9; LAP-2; HSP89A; HSP9A; HSP9N; HSPCAL1; HSPCAL4; Heat shock protein HSP 9-alpha; Heat shock 86 kDa; HSP 86; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; HSP9AA1
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-10100R Datasheet
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DELIVERY
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