TLR2 Polyclonal Antibody
Catalogue Number: BS-1019R-BSS
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Mouse spleen cells probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
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Lane 1: Mouse Spleen; Lane 2: Mouse Thymus; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Lane 1: Raw264.7 Cells; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4°C followed by incubation with a conjugated secondary antibody for 60 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
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Lane 1: Cerebrum lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Rat Spleen lysates; Lane 2: Rat Bronchus lysates; Lane 3: Rat Lung lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Human A549 cells probed with Rabbit Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | TIL4; CD282; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; TLR2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TLR2 (cytoplasmic domain) |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Additional Text
Gene Name
TLR2
Gene ID
7097
Uniprot ID
O60603
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1019R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations