NSE Polyclonal Antibody
Catalogue Number: BS-1027R-BSS
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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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A431 lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Lane 1: Mouse brain lysates; Lane 2: Mouse cerebellum lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Lane 1: Mouse Spinal cord lysates; Lane 2: Rat Spinal cord lysates probed with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200 overnight at 4°C, DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NSE Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200 overnight at 4°C, DAB staining.
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Neuroblastoma cells labeled with Anti-NSE/ENO2/γ Enolase Polyclonal Antibody, Unconjugated (bs-1027R) at 1:200, followed by conjugation to the secondary antibody
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Formalin-fixed and paraffin embedded dog bladder tissue labeled with Anti-NSE/ENO2/γ Enolase Polyclonal Antibody, Unconjugated (bs-1027R) at 1:800, followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NSE; HEL-S-279; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; ENO2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human NSE |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Additional Text
Gene Name
ENO2
Gene ID
2026
Uniprot ID
P09104
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1027R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations