TMP21 Polyclonal Antibody
Catalogue Number: BS-11262R-BSS
BS-11262R Image
BS-11262R Image
BS-11262R Image
BS-11262R Image
BS-11262R Image
BS-11262R Image
BS-11262R Image
BS-11262R Image
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with TMP21 Polyclonal Antibody, Unconjugated (bs-11262R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BS-11262R Image
Lane 1: Mouse Pancreas lysates; Lane 2: Mouse Large intestine lysates; Lane 3: Mouse Placenta lysates probed with TMP21 Polyclonal Antibody, Unconjugated (bs-11262R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-11262R Image
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Placenta lysates probed with TMP21 Polyclonal Antibody, Unconjugated (bs-11262R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-11262R Image
Lane 1: Human HEK293T KO TMP21 cell lysates; Lane 2: Human HEK293T cell lysates probed with TMP21 Polyclonal Antibody, Unconjugated (bs-11262R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-11262R Image
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (bs-11262R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-11262R Image
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (bs-11262R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BS-11262R Image
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human Huvec cell lysates; Lane 5: Human THP-1 cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human HL-60 cell lysates probed with TMP21 Polyclonal Antibody, Unconjugated (bs-11262R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TMP21 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: MP21 is a member of the EMP24/GP25L/p24 cargo family of proteins that regulates vesicular trafficking in the early secretory pathway. TMP21 is a ubiquitously expressed single-pass type I membrane protein localizing to the Golgi cisternae and the plasma membrane. It contains one GOLD (Golgi dynamics) domain and participates in protein transport and quality control between the endoplasmic reticulum (ER) and the Golgi complex. In addition, TMP21 is a component of the heteromeric secretase complex (or Presenilin complex) and functions to regulate the ?secretase activity. Two isoforms, namely TMP21-I and TMP21-II, exist for this protein.
Additional Text
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
p23; p24 family protein delta 1; p24delta; p24delta1; S31I125; S31III125; TMED 10; TMED10; Tmp 21 I; Tmp 21; Tmp 21 I; Transmembrane emp24 domain containing protein 10; Transmembrane emp24-like trafficking protein 10 yeast; Transmembrane protein Tmp21; Transmembrane trafficking protein 21kD; 1110014C03Rik; 21 kDa transmembrane trafficking protein; MGC102351; TMEDA_HUMAN.
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-11262R Datasheet
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DELIVERY
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