CD28 Polyclonal Antibody
Catalogue Number: BS-1297R-BSS
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Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
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Lane 1: Thymus lysates probed with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (bs-1297R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | T-cell-specific surface glycoprotein CD28; CD28 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse CD28 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Additional Text
Gene ID
12487
Gene Name
CD28
Uniprot ID
P31041
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1297R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations