CD15/Fut4/SSEA-1 Polyclonal Antibody
Catalogue Number: BS-1702R-BSS
bs-1702R Image
bs-1702R Image
bs-1702R Image
bs-1702R Image
Human HL-60 cells probed with CD15 Polyclonal Antibody, Unconjugated (bs-1702R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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Lane 1: MCF-7 lysates probed with CD15/Fut4/SSEA-1 Polyclonal Antibody, Unconjugated (bs-1702R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37°C.
bs-1702R Image
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD15/Fut4/SSEA-1 Polyclonal Antibody(bs-1702R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | FAL; LeX; CD15; Ssea1; SSEA-1; FucT-IV; AI451562; Alpha-(1,3)-fucosyltransferase 4; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; Galactoside 3-L-fucosyltransferase; Fut4; Elft |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse CD15 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Additional Text
Gene Name
FUT4
Gene ID
14345
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Uniprot ID
Q11127
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1702R Datasheet
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