PD-1/CD279 Polyclonal Antibody
Catalogue Number: BS-1867R-BSS
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Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 30kD. Observed band size:30kD.
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Lane 1: Hela Cell lysates; Lane 2: Molt-4 Cell Lysates; Lane 3: Jurkat Cell Lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-1867R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Mouse spleen cells were fixed with 70% ice-cold methanol overnight at 4°C and then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions. The cells were then probed with rabbit anti-PD-1 Polyclonal Antibody, with PE-CY7 conjugation (bs-1867R-PE-CY7) (green) at 1:100 for 30 minutes at room temperature. The graph compares PD-1 antibody to unstained cells (blue) and isotype control (orange).
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Lane 1: Rat Raji cell lysates; Lane 2: Mouse Thymus lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Spleen lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Rat Thymus lysates probed with PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PD-1 ) Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PD-1 ) Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; Programmed cell death protein 1; Protein PD-1; PDCD1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PD-1 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
Additional Text
Gene Name
PDCD1
Gene ID
5133
Uniprot ID
Q15116
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1867R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations