PI3KCA Polyclonal Antibody
Catalogue Number: BS-2067R-BSS
bs-2067R Image
bs-2067R Image
bs-2067R Image
bs-2067R Image
bs-2067R Image
bs-2067R Image
bs-2067R Image
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
bs-2067R Image
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
bs-2067R Image
HeLa Cell lysates probed with PI3KCA Polyclonal Antibody, unconjugated (bs-2067R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
bs-2067R Image
Lane 1: SW480 cell lysates; Lane 2: NIH/3T3 cell lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-2067R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bs-2067R Image
Lane 1: Human SW480 cell lysates; Lane 2: Human Molt-4 cell lysates; Lane 3: Human HL60 cell lysate probed with PIK3CA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bs-2067R Image
NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PIK3CA) polyclonal Antibody, Unconjugated (bs-2067R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PI3KCA |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.
Additional Text
Gene ID
5290
Gene Name
PIK3CA
Uniprot ID
P42336
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p11-alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p11-alpha; p11alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA; PIK3CA
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-2067R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations