GABA A Receptor gamma 2 Antibody
Catalogue Number: BS-4112R-BSS
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Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded rat brain labeled with Anti GABRG2/GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GABA A Receptor gamma 2) polyclonal Antibody, Unconjugated (bs-4112R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti GABRG2/GABA A Receptor gamma 2 Polyclonal Antibody, Unconjugated (bs-4112R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 51kD. Observed band size: 51kD.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CAE2; ECA2; GEFSP3; Gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; GABRG2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human GABRG2/GABA A Receptor gamma 2 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
Additional Text
Gene Name
GABRG2
Gene ID
2566
Uniprot ID
P18507
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-4112R Datasheet
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