CD161c/NK1.1 Polyclonal Antibody
Catalogue Number: BS-4682R-BSS
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bs-4682R Image
bs-4682R Image
bs-4682R Image
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Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD161c/NK1.1 Polyclonal Antibody, Unconjugated (bs-4682R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
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Lane 1: Human U937 cell Lysates; Lane 2: Human Jurkat cell Lysates; Lane 3: Human MOLT4 cell Lysates; Lane 4: Human MCF-7 cell Lysates. Probed with CD161c polyclonal Antibody, unconjugated (bs-4682R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Mouse plasma probed with Rabbit Anti-CD161c Polyclonal Antibody, Unconjugated (bs-4682R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
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Lane 1: mouse intestine lysates Lane 2: mouse liver lysates probed with Anti CD161c/NK1.1 Polyclonal Antibody, Unconjugated (bs-4682R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 25kD. Observed band size: 25kD.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse CD161/NK1.1 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB |
Description
Description: Plays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.
Additional Text
Gene ID
17059
Gene Name
Klrb1c
Purification
Protein A purified
Uniprot ID
P27814
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Specificity
Nk1; Ly59; Nk-1; CD161; Ly-59; Ly55c; NK1.1; NKRP1; Nk1.2; NK-RP1; Nk-1.2; Nkrp1c; ly-55c; NKRP14; AI462337; NKR-P1.9; Killer cell lectin-like receptor subfamily B member 1C; CD161 antigen-like family member C; Lymphocyte antigen 55c; NKR-P1C; Natural killer cell surface protein P1-4; NKR-P1 4; CD161c; Klrb1c
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-4682R Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations