H3K9me1 Polyclonal Antibody
Catalogue Number: BS-53043R-BSS
BS-53043R Image
BS-53043R Image
BS-53043R Image
BS-53043R Image
BS-53043R Image
BS-53043R Image
HeLa cells were stained with the H3K9me1 antibody (bs-53043R) and with DAPI. Cells were formaldehyde fixated, permeabilized with Triton X-100 and blocked with PBS containing 2.5% BSA. Figure A: cells were immunofluorescently labelled with the H3K9me1 antibody (diluted 1:200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to DyLight. Figure B: staining of the nuclei with DAPI, which specifically labels DNA. Both antibody and DAPI staining are restricted to the nucleus.
BS-53043R Image
To determine the titer, an ELISA was performed using a serial dilution of the H3K9me1 antibody (bs-53043R). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:50,000.
BS-53043R Image
A Dot Blot analysis was performed to test the cross reactivity of H3K9me1 antibody (bs-53043R) with peptides containing other modifications of histone H3. Other histone modifications include di- and trimethylation of the same lysine and mono-, di- and trimethylation of lysine 27, 36 and 79. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:200,000. The figure shows a high specificity of the antibody for the modification of interest.
BS-53043R Image
Histone extracts of HeLa cells (15 µg) were analysed by Western blot using the H3K9me1 antibody (bs-53043R) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.
BS-53043R Image
ChIP assays were performed using U2OS cells, the antibody against H3K9me1 (bs-53043R) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 2, 5, 10 and 15 µl per ChIP experiment was analyzed. IgG (5 µg/IP) was used as negative IP control. Quantitative PCR was performed using primers for the promoter of the housekeeping gene GAPDH and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive under normal conditions. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Preservative: | 0.05% Sodium azide |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | Polyclonal antibody raised in rabbit against histone H3 containing the monomethylated lysine 9 (H3K9me1), using a KLH-conjugated synthetic peptide |
| Application: | ELISA, IF, WB, DB |
Description
Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Text
Uniprot ID
P68431
Gene ID
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Antibody Clonality
Polyclonal
Modification
Unmodified
Gene Name
H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8
Storage Note
Store at -20°C for 12 months.
bs-53043R Datasheet
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