Beclin 1 Polyclonal Antibody
Catalogue Number: BS-55024R-BSS
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
BS-55024R Image
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:100 overnight at 4°C, DAB staining.
BS-55024R Image
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:100 overnight at 4°C, DAB staining.
BS-55024R Image
Lane 1: U-87MG cell lysates; Lane 2: Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Mouse Spleen lysates; Lane 5: Rat ovary lysates; Lane 6: Rat Lung cell lysates probed with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-55024R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BS-55024R Image
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, DAB staining.
BS-55024R Image
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, DAB staining.
BS-55024R Image
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, DAB staining.
BS-55024R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, DAB staining.
BS-55024R Image
Lane 1: Human 293T cell lysates; Lane 2: Human 293T KO Beclin 1 cell lysates probed with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55024R Image
Lane 1: Mouse Cerebrum tissue lysates;Lane 2: Mouse Small intestine tissue lysates;Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse Heart tissue lysates ; Lane 5: Rat Cerebrum tissue lysates;Lane 6: Human Jurkat cell lysates; Lane 7: Human Raji cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human HepG2 cell lysates probed with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ATG6;VPS30;beclin1;Beclin 1;BECN1;beclin-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Additional Text
Gene Name
BECN1
Gene ID
8678
Uniprot ID
Q14457
Purification
Protein A purified
Concentration
0.5 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-55024R Datasheet
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DELIVERY
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