DNMT1 Polyclonal Antibody
Catalogue Number: BS-55056R-BSS
BS-55056R Image
BS-55056R Image
BS-55056R Image
BS-55056R Image
bs-55056R Image
BS-55056R Image
Lane 1: 293T cell lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55056R Image
293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DNMT1) polyclonal Antibody, Unconjugated (bs-55056R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
BS-55056R Image
Lane 1: Human Jurkat cell lysates; Lane 2: Human Molt-4 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human 293T cell lysates ; Lane 5: Human U251 cell lysates ; Lane 6: Human HL60 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BS-55056R Image
Lane 1: Human 293T cell (Control) lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bs-55056R Image
Lane 1: 293T cell lysates; Lane 2: SW480 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1). |
| Application: | ICC, IF, IP, WB |
Description
Description: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Additional Text
Gene Name
DNMT1
Uniprot ID
P26358
Gene ID
1786
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-55056R Datasheet
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DELIVERY
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