His tag (2D5) Monoclonal Antibody
Catalogue Number: BSM-0287M-BSS
BSM-0287M Image
BSM-0287M Image
Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-His tag (bsm-0287M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Tag antibodies |
| Alias: | HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; 6 HIS-TAG; 6 HIS TAG; 6-HIS-TAG; Histidine. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 2D5 |
| Isotype: | IgG |
| Immunogen: | KLH conjugated HHHHHH peptide |
| Application: | WB |
Description
Description: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Additional Text
Purification
Protein G purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-0287M Datasheet
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