FOXA1 Monoclonal Antibody
Catalogue Number: BSM-52072R-BSS
BSM-52072R Image
BSM-52072R Image
BSM-52072R Image
bsm-52072R Image
BSM-52072R Image
Paraformaldehyde-fixed, paraffin embedded mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52072R Image
MCF-7 cells were stained with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52072R Image
Lane 1: Human MCF-7 cell Lysates; Lane 2: Human HepG2 cell Lysates. Probed with FOXA1 monoclonal Antibody, unconjugated (bsm-52072R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bsm-52072R Image
Lane 1: 293 lysates; Lane 2: Mouse lung lysates probed with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | HNF3A; TCF3A; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3A; FOXA1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 5F7 |
| Isotype: | IgG |
| Immunogen: | Recombinant human FOXA1 protein |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
Additional Text
Gene Name
FOXA1
Gene ID
3169
Uniprot ID
P55317
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52072R Datasheet
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