STAT3(S727) (4G1) Monoclonal Antibody
Catalogue Number: BSM-52210R-BSS
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
BSM-52210R Image
bsm-52210R Image
BSM-52210R Image
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52210R Image
A431 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52210R Image
NIH/3T3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
BSM-52210R Image
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with STAT3(S727) (4G1) Monoclonal Antibody (bsm-52210R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52210R Image
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with STAT3(S727) (4G1) Monoclonal Antibody (bsm-52210R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52210R Image
Paraformaldehyde-fixed, paraffin embedded rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with STAT3(S727) (4G1) Monoclonal Antibody (bsm-52210R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
BSM-52210R Image
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with STAT3(S727) (4G1) Monoclonal Antibody(bsm-52210R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
BSM-52210R Image
Lane 1:Rat Heart lysates; Lane 2:Rat Cerebrum lysates probed with phospho-STAT3 (Ser727) Monoclonal Antibody, Unconjugated (bsm-52210R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52210R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3(S727) (4G1) Monoclonal Antibody, Unconjugated (bsm-52210R) at 1:200 overnight at 4°C, DAB staining.
BSM-52210R Image
Lane 1: Human Hela cell lysates; Lane 2: Human A549 cell lysates probed with STAT3(S727) (4G1) Monoclonal Antibody, Unconjugated (bsm-52210R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-52210R Image
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-STAT3 (Ser727)) monoclonal Antibody, Unconjugated (bs-52210R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
bsm-52210R Image
Lane 1: HeLa lysates; Lane 2: NIH/3T3 lysates; probed with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Ph: | 7.4 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | APRF; HIES; ADMIO; Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 4G1 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide derived from human STAT3(S727), around 700-750aa (phospho S727). |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).
Additional Text
Gene Name
STAT3
Gene ID
6774
Uniprot ID
P40763
Modification
Phosphorylated, Ser727
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bsm-52210R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations