CaMKII (1H12) Monoclonal Antibody
Catalogue Number: BSM-52427R-BSS
BSM-52427R Image
BSM-52427R Image
BSM-52427R Image
BSM-52427R Image
BSM-52427R Image
bsm-52427R Image
BSM-52427R Image
IF(ICC) staining with CaMKII (1H12) Monoclonal Antibody (bsm-52427R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52427R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CaMKII (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52427R Image
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CaMKII (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52427R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse cerebellum tissue incubated with CaMKII (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52427R Image
Paraformaldehyde-fixed and paraffin-embedded Rat cerebellum tissue incubated with CaMKII (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
bsm-52427R Image
Lane 1: SH-SY-5Y cell; Lane 2: PC-12 cell; Lane 3: SHG-44 cell; Probed with CaMKⅡ (1H12) Monoclonal Antibody (bsm-52427R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 1H12 |
| Isotype: | IgG |
| Immunogen: | Human CaMKII between 200-300 amino acids |
| Application: | ICC, IF, IHC-P, WB, IHC |
Description
Description: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
Additional Text
Gene Name
CAMK2B
Gene ID
816
Uniprot ID
Q13554
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Specificity
Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit beta, Calcium/calmodulin-dependent protein kinase type II subunit gamma, Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968, CaM kinase II subunit beta, CaMK-II subunit beta, CAMK2B, CAM2, CAMK2, CAMKB, CaM kinase II subunit gamma, CaMK-II subunit gamma, CAMK2G, CAMK, CAMK-II, CAMKG, CaM kinase II subunit delta, CaMK-II subunit delta, CAMK2D, CAMKD
Storage Note
Store at -20°C for 12 months.
bsm-52427R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations