14-3-3 alpha+beta (3C5) Monoclonal Antibody
Catalogue Number: BSM-52710R-BSS
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
BSM-52710R Image
Flow cytometric analysis of HeLa cells with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-52710R Image
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Probed with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52710R Image
IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52710R Image
IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52710R Image
IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52710R Image
IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52710R Image
Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52710R Image
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52710R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52710R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, YWHAB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 3C5 |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to C terminal human 14-3-3 alpha + beta |
| Application: | FC, ICC, IF, IHC-P, WB, IHC |
Description
Description: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Additional Text
Gene Name
YWHAB
Gene ID
7529
Uniprot ID
P31946
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52710R Datasheet
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