CRISPR Cas9 Protein (CAS-EE109)
Catalogue Number: CAS-EE109-KAT
CAS-EE109 Image
CAS-EE109 Image
CAS-EE109 Image
CAS-EE109 Image
CAS-EE109 Image
Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of KACTUS Cas9 is equivalent to that of leading competitors.
CAS-EE109 Image
Figure 2. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show KACTUS Cas9 has the same knockout efficiency as leading competitors.
CAS-EE109 Image
Figure 3. The purity of KACTUS Cas9 detected by SEC-HPLC is greater than 95%.
CAS-EE109 Image
Figure 4. The purity of KACTUS Cas9 detected by Tris-Bis-PAGE is greater than 95%.
| Manufacturer: | Kactus |
| Physical state: | Liquid |
| Type: | Enzyme |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 1 mg |
Description
Description: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9.
Additional Text
Storage Note
-20+/-5°C for 24 months. Avoid repeated freeze-thaw cycles.
CAS-EE109 Datasheet
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