Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)
Catalogue Number: CD3-HM157-KAT
CD3-HM157 Image
CD3-HM157 Image
CD3-HM157 Image
CD3-HM157 Image
CD3-HM157 Image
CD3-HM157 Image
Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22nM as determined in SPR assay (Biacore T200).
CD3-HM157 Image
Immobilized Human CD3E&CD3G, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2ng/ml determined by ELISA.
CD3-HM157 Image
Immobilized Human CD3E&CD3G, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2ng/ml determined by ELISA.
CD3-HM157 Image
The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.
CD3-HM157 Image
Human CD3E&CD3G on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Alias: | CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
Description
Description: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Additional Text
Gene ID
916
Protein Tag
HIS Tag
Uniprot ID
P07766, P09693
Short Description
Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E)&Gln23-Asn106(CD3G).
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Gene Name
CD3E, CD3G
CD3-HM157 Datasheet
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