5-TCO-PEG4-dUTP, 5-trans-Cyclooctene-PEG4-dUTP
Catalogue Number: CLK-035S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Weight: | 922.70 g/mol (free acid) |
| Molecular Formula: | C32H53N4O21P3 (free acid) |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 200 ul |
Description
Description: 5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasksto introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA byPCR with Taq polymerase in-house data
Smile String
C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCOCCOCCOCCOCCNC(=O)OC1CCCC=CCC1
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-035S Datasheet
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