5-DBCO-PEG4-dUTP
Catalogue Number: CLK-048-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Weight: | 1057.83 g/mol (free acid) |
| Molecular Formula: | C42H54N5O21P3 (free acid) |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 50 ul |
Description
Description: 5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasksto introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Additional Text
Concentration
10-11 mM
Application Notes
Incorporation into DNA/cDNA byPCR with Taq polymerase in-house data
Short Description
5-Dibenzylcyclooctyl-PEG4-uridine-5'-triphosphate, Triethylammonium salt
Smile String
c1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-048-S Datasheet
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