5-Azidomethyl-2'-deoxyuridine (5-AmdU)
Catalogue Number: CLK-064-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | RT |
| Unit(s): | 10 mg |
Description
Description: 5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine. The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice tointroduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly)introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).
Additional Text
Solubility
DMSO, methanol
Application Notes
DNA synthesis monitoring[1]
Smile String
O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](CO)[C@@H](O)C1)CN=[N+]=[N-]
CLK-064 MSDS
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