Catalogue Number: CLK-076-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Physical state: | solid |
Type: | Conjugation Reagent |
Shipping Condition: | Blue Ice |
Unit(s): | 0.5 mg |
Application: | Cnjg |
water, aqueous buffer such as PBS
[C@H]1([C@H](C(O[C@@H]([C@H]1O)CN=[N+]=[N-])OP(=O)(OP(=O)(OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)cc1)O)O)O)O
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 β-Glycosyltransferase (T4 β-GT)[1-3] The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice to introduce a (Desthio)Biotin group for subsequent purification tasks (via DBCO-containing (Desthio)Biotin)to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes) 0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 µl of a 3 mM solution.
Sodium salt UDP-6-N3-Glc, 6-N3-UDPG