5-Ethynyl-2'-deoxyuridine (5-EdU)
Catalogue Number: CLK-N001-25-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Formula: | C11H12N2O5 |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | RT |
| Unit(s): | 25 mg |
Description
Description: 5-EdU (5-Ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging [1-5]. Presolski et al.[6] and Hong et al.[7] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Solubility
DMSO, up to 200 mM (at room temperature) in 1 x PBS or water by heating the obtained suspension for 1 minute by 70 °C
Application Notes
DNA synthesis monitoring[1-5]
Smile String
n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O
Short Description
5-Ethynyl-2'-deoxyuridine
Storage Note
store at -20 °C|12 months after date of delivery|store dry and under inert gas|Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-N001-25 Datasheet
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