2-Ethynyl-ATP (2-EATP)
Catalogue Number: CLK-NU-004L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Weight: | 531.20 g/mol |
| Molecular Formula: | C12H16N5O13P3 |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 x 100 ul |
Description
Description: 2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitro polyadenylation of RNA with recombinant poly(A) polymerase[1]. The resulting Alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed Azide-Alkyne click chemistry (CUAAC) that offers the choice to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)to crosslink the RNA to azide-functionalized biomolecules e.g.proteins Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Concentration
10-11 mM
Application Notes
in vitro polyadenylation of RNA[1]
Short Description
2-Ethynyl-adenosine-5'-triphosphate, Sodium salt
Smile String
[C@@H]1([C@@H](O[C@@H]([C@H]1O)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1cnc2c(nc(nc12)C#C)N)O
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-NU-004L Datasheet
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