C8-Alkyne-dUTP
Catalogue Number: CLK-T05-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 x 5 ul |
| Application: | Cnjg |
Additional Text
Concentration
100-110 mM
Application Notes
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choiceto introduce a Biotin group (via Azides of Biotin) for subsequent purification tasksto introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Azide-functionalized biomolecules e.g.proteins Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Short Description
5-(Octa-1,7-diynyl)-2'-deoxyuridine 5'-triphosphate, Sodium salt
Smile String
c1(=O)[nH]c(=O)n(cc1C#CCCCCC#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
Molecular Weight
572.29 g/mol (free acid)
Molecular Formula
C17H23N2O14P3 (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-T05-L Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations