5-Ethynyl-UTP (5-EUTP)
Catalogue Number: CLK-T08-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Weight: | 508.16 g/mol (free acid) |
| Molecular Formula: | C11H15N2O15P3 (free acid) |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 ul |
| Application: | Cnjg |
Additional Text
Concentration
100-110 mM
Application Notes
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choiceto introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)to crosslink the RNA to azide-functionalized biomolecules e.g.proteins Presolski et al.[1] and Hong et al.[2] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Short Description
5-Ethynyl-uridine-5'-triphosphate, Sodium salt
Smile String
n1([C@@H]2O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@H]([C@H]2O)O)c(=O)[nH]c(=O)c(c1)C#C
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-T08-S Datasheet
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