γ-[(6-Azidohexyl)-imido]-ATP
Catalogue Number: CLK-T12-1-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Formula: | C16H28N9O12P3 (free acid) |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were γ-[(6-Azidohexyl)-imido]-ATP (compound 6[1]) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Solubility
10 mM Tris-HCl pH 7.5
Application Notes
in vitro phosphorylation of recombinant proteins[1]
Short Description
γ-[(6-Azidohexyl)-imido]-adenosine-5'-triphosphate, Sodium salt
Smile String
O[C@H]1[C@H](n2cnc3c(N)ncnc23)O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)NCCCCCCN=[N+]=[N-])[C@H]1O
Additional Information
Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
CLK-T12-1 Datasheet
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