JBS True Blue
Catalogue Number: CO-301-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Biological dyes |
| Shipping Condition: | RT |
| Storage Condition: | RT |
| Unit(s): | 300 ul |
Description
Description: Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals. Staining of crystals with appropriate dyes is a very straightforward method to differentiate between macromolecular crystals and salt crystals [1]. Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like JBS True Blue, are able to permeate the solvent channels of a protein, thus coloring the protein blue. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.
Additional Text
Application Notes
JBS True Blue is a crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.
Short Description
Methylene Blue hydrated
CAS Number
61-73-4
Molecular Weight
319.85 g/mol (anhydrous)
Molecular Formula
C16H18ClN3S * H2O
Smile String
[Cl-].s1c2c(nc3c1cc(N(C)C)cc3)ccc(=[N+](C)C)c2
CO-301 MSDS
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