GST Monoclonal Antibody

Catalogue Number: CSB-MA000031M0M-CSB

Manufacturer:Cusabio Biotech
Preservative:0.03% ProClin™ 300
Ph:7.4
Physical state:Liquid
Type:Monoclonal Primary Antibody - Unconjugated
Shipping Condition:Blue Ice
Unit(s): 100 ug, 50 ug
Host name: Mouse
Clone:
Isotype: IgG2a
Immunogen: Recombinant GST Protein
Application: ELISA, WB

Description

Description: Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

Additional Text

Purification

Protein G purified

Antibody Clonality

Monoclonal