MBP Monoclonal Antibody
Catalogue Number: CSB-MA000061M0M-CSB
CSB-MA000061M0M Image
CSB-MA000061M0M Image
CSB-MA000061M0M Image
MBP-tagged fusion protein(20ng/ml) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000061M0m at dilution of Lane 1:1000 Lane 4:8000 Lane 7:64000 Lane 2:2000 Lane 5:16000 Lane 8:128000 Lane 3:4000 Lane 6:32000 Lane 9:256000 Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size:50kd Observed band size:50kd
CSB-MA000061M0M Image
MBP-tagged fusion protein(20ng/ml) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000061M0m at dilution of Lane 1:1000 Lane 4:8000 Lane 7:64000 Lane 2:2000 Lane 5:16000 Lane 8:128000 Lane 3:4000 Lane 6:32000 Lane 9:256000 Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size:50kd Observed band size:50kd
| Manufacturer: | Cusabio Biotech |
| Preservative: | 0.03% ProClin™ 300 |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG2a |
| Immunogen: | MBP-Tag fusion protein |
| Application: | ELISA, WB |
Description
Description: Maltose-Binding Protein (MBP) is a native E. coli protein and is part of a complex responsible for the uptake and efficient catabolism of maltodextrins. MBP is composed of 370 amino acid residues. As a fusion tag, MBP is useful for recombinant protein purification by affinity chromatography. It is also used to increase the expression level and solubility of the tagged protein, and for promoting proper folding of the attached protein. MBP increases solubility and the tag is thus particularly useful for recombinant proteins that may otherwise accumulate in an insoluble forms as inclusion bodies. Affinity purification takes place under physiological conditions and mild elution is performed using maltose. The mild elution conditions preserve the activity of the MBP-tagged protein.
Additional Text
Purification
Protein G purified
Antibody Clonality
Monoclonal
CSB-MA000061M0m Datasheet
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