GAPDH Monoclonal Antibody

Catalogue Number: CSB-MA000071M2M-CSB

CSB-MA000071M2M Image

Western Blot Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time : 5min

CSB-MA000071M2M Image

Western Blot Positive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time : 5min

CSB-MA000071M2M Image

Western Blot Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat spleen tissue, Rat brain tissue, Rat skeletal tissue, Rat stomach tissue, Rat kidney tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time : 10S

CSB-MA000071M2M Image

Western Blot Positive WB detected in: Rabbit heart tissue, Rabbit kidney tissue, Rabbit skeletal muscle tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time : 5min

CSB-MA000071M2M Image

Western Blot Positive WB detected in: Hela whole cell lysate at 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µg, 0.15625µg, 0.078µg All lanes:GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time : 5min

CSB-MA000071M2M Image

Western Blot Positive WB detected in: 15µg hela whole cell lysate GAPDH antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure timeL: 5min

CSB-MA000071M2M Image

IHC image of CSB-MA000071M2m diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-MA000071M2M Image

IHC image of CSB-MA000071M2m diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-MA000071M2M Image

IHC image of CSB-MA000071M2m diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

CSB-MA000071M2M Image

Immunofluorescence staining of Hela cells with (CSB-MA000071M2m) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

CSB-MA000071M2M Image

Immunofluorescence staining of HepG2 cells with (CSB-MA000071M2m) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

CSB-MA000071M2M Image

Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000071M2m in Hela whole cell lysate. Lane 2: CSB-MA000071M2m (5µl) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg) For western blotting, the blot was detected with CSB-MA000071M2m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

CSB-MA000071M2M Image

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Manufacturer:	Cusabio Biotech
Preservative:	0.03% ProClin™ 300
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 50 ul
Host name:	Mouse
Clone:	10H4D6
Isotype:	IgG1
Immunogen:	Recombinant Human GAPDH protein (2-335AA)
Application:	ELISA, IF, IP, WB, IHC

Overview

Description

Description: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.