CD44 Monoclonal Antibody

Catalogue Number: CSB-MA004938A0M-CSB

CSB-MA004938A0M Image

Western Blot Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate All lanes: CD44 antibody at 1:1500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa Observed band size: 95 kDa

CSB-MA004938A0M Image

Western Blot Positive WB detected in: A549 whole cell lysate All lanes: CD44 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa Observed band size: 95 kDa

CSB-MA004938A0M Image

IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-MA004938A0M Image

IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-MA004938A0M Image

IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CSB-MA004938A0M Image

Immunofluorescence staining of Hela cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA004938A0M Image

Immunofluorescence staining of MCF-7 cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA004938A0M Image

Overlay histogram showing Hela cells stained with CSB-MA004938A0m (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

CSB-MA004938A0M Image

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Manufacturer:	Cusabio Biotech
Preservative:	0.03% ProClin™ 300
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 50 ul
Host name:	Mouse
Clone:	6G2D2
Isotype:	IgG1, kappa
Immunogen:	Recombinant Human CD44 antigen protein (21-220AA)
Application:	ELISA, FC, IF, WB, IHC

Overview

Description

Description: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity).

Additional Text

Gene Name

CD44

Gene ID

960

Uniprot ID

P16070

Purification

Protein G purified

Specificity

CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4