Rhamnogalacturonan endolyase 9B, Bacteroides thetaiotaomicron

Catalogue Number: CZ01862-NZY

Manufacturer:NZYTech
Type:Other Enzymes
Host Cell:E.coli
Shipping Condition:RT
Unit(s): 3 x 1 mg

Description

Description: Rhamnogalacturonan endolyase 9B (BtRge9B), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9B, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.

Additional Text

Purification

Purified

Short Description

Rhamnogalacturonan endolyase 9B (BtRge9B), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9B, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).