Total Bile Acid (TBA) Colorimetric Assay Kit

Catalogue Number: E-BC-K181-ELA

Manufacturer:	Elabscience
Shelf Life:	6 months
Type:	Detection Kit
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 assays
Range:	0-180 µmol/L
Sensitivity:
Sample type:	Serum
Sample size:

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Datasheets

Description

Description: Application The kit is used for the quantitative determination of the total bile acid concentration in serum. Detection significance Total bile acid (TBA) is mainly used for the screening and prognosis of follow-up of hepatobiliary disease and as the marker of liver parenchymal damage and cholestasis. The increase of TBA indicates the risk of viral hepatitis, cirrhosis, alcoholic liver disease, drug-induced liver injury or cholestasis. Detection principle Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid. Sample requirements 1. Separate serum within 2 hours after blood collection. The serum sample can be stored at 15~30°C within 8 hours, at 2~8°C for a week or at -20°C for 3 months. 2. Interfering substances: conjugated bilirubin≤ 5mg/dL, unconjugated bilirubin ≤ 20mg/dL, vitamin C ≤ 1mg/dL, glycerin trilaurate ≤ 9.25 mmol/L, hemoglobin ≤ 100mg/dL have no effect to the results. Operation steps 1. Detection with Spectrophotometer Operation table 2. Detection with Microplate reader Operation table 3. Detection with Biochemical analyzer Setting parameter Measure the absorbance at 0 second (A1) and 120 second (A2), respectively. Calculate the A/min. Automatic biochemical analyzer has its own program parameter input language. Reagents matches the analyzer and carry out automatic measurement after the above basic parameters are modified. Reference range 1.2~10.5 µmol/L (This data is for reference only. It is recommended that each laboratory establish its own range of reference values.) Technical parameter 1. Linear range: 0 ~ 150 µmol/L, r2 ≥ 0.990. 2. Accuracy: inaccuracy ≤ 15.0%. 3. Recovery rate: 100 +/- 20% 4. Precision: intra-CV ≤ 5.0%, inter-CV ≤ 10.0%. 5. Absorbance for the blank control (reagents only) ≤ 0.7 (405 nm wavelength, 1 cm optical path). Notes 1. Instructions should be followed strictly, changes of operation may result in unreliable results. 2. The validity of kit is 12 months. 3. Do not use components from different batches of kit. 4. The sample needs to be diluted with normal saline before the determination when the concentration of TBA is higher than 150 µmol/L. The result should be multiplied by the dilution factor. 5. The kit is for research use only and contains preservatives. It should be avoided to contact with skin and clothing. Wash immediately with plenty of water if contact it carelessly. 6. The ratio of sample and reagent can be scaled as required.

Additional Text

2-8°C

E-BC-K181 Protocol

Price

£244.00

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