Proteinase K Reagent(10 mg/mL)
Catalogue Number: E-IR-R109-ELA
| Manufacturer: | Elabscience |
| Type: | Proteases |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 ml, 10 ml, 5 ml |
| Application: | FC, IF |
Description
Description: Introduction Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on. Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity. The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0. The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55 °C. Components Specification parameters Storage Store at -20°C. Valid for 12 months.
Additional Text
-20°C
E-IR-R109 Protocol
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations