Phos binding reagent (Phosbind) Biotin

Catalogue Number: F4001-APE

Manufacturer:	Apexbio
Molecular Formula:	C40H50N10O4S
Type:	Other Reagents
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	10 mg, 5 mg, 50 mg

Overview
Datasheets

Description

Description: Phosbind Biotin provides a sensitive method for detection of phosphorylated proteins on a PVDF membrane. This method needs streptavidin-conjugated horseradish (HRP) and chemiluminescent detection reagent.Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.The determination of the phosphorylation state of proteins is important for defining protein kinase substrates and revealing the activation state of signal transduction pathways. Methods for determining the phosphorylation status of proteins are thus have important implications in the understanding of diverse biological and pathophysiological processes, such as signal transduction pathways, cancer and other diseases.Phos binding reagents (Phosbind) are specific and selective phosphate-binding reagents, and exhibit preferential ionic interactions with phosphorylated ions on phosphorylated proteins or peptides at neutral pH. Phos binding reagent is a dinuclear metal (Zn2+ or Mn2+) complex.Phosbind Biotin is a specific reagent for detection of phosphorylated proteins using Western Blot and can instead of the specific phosphorylated antibodies.

Additional Text

Short Description

Detection of phosphorylated proteins

Solubility

insoluble in H2O; insoluble in EtOH; ≥32.3 mg/mL in DMSO

Storage Note

2-10°C

F4001 MSDS

Price

£400.00

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