Catalogue Number: F4005-APE
Manufacturer: | Apexbio |
Type: | Prestained Protein Molecular Weight Markers |
Shipping Condition: | Blue Ice |
Unit(s): | 250 ul, 2 x 250 ul |
Description: The Prestained Protein Marker (Triple color, EDTA free, 10-250 kDa) is a prestained mixture of ten recombinant proteins ranging from 10 kDa to 250 kDa. Three different chromophores are bound to the proteins, 9 bands with blue, one red at 70 kDa and one green at 25 kDa. The Prestained Protein Marker is applied for observing protein separation during SDS-PAGE electrophoresis, verification of western transfer efficiency on membranes, and identifying the size of proteins. The marker can also be used in fluorescent imaging of membranes.Prestained Protein Marker obtained increased brightness providing visual robustness throughout western blotting workflow, to the benefit of identifying target proteins on the gel or membrane, monitoring gel electrophoresis and confirming transfer quality.The buffer of the marker doesn't contain EDTA which means it can be used in Phosbind SDS-PAGE electrophoresis.The recommended volume for 1mm mini-gel is 3-5 µl. Large size gels need more volume.