Human GFRAL/GFR alpha-like Protein (GFL-HM201)
Catalogue Number: GFL-HM201-KAT
GFL-HM201 Image
GFL-HM201 Image
GFL-HM201 Image
GFL-HM201 Image
GFL-HM201 Image
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).
GFL-HM201 Image
Immobilized Human GDF15, His Tag at 0.5µg/ml (100µl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
GFL-HM201 Image
The purity of Human GFRAL is greater than 95% as determined by SEC-HPLC.
GFL-HM201 Image
Human GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Alias: | GFR alpha-like; GFRAL; GRAL; C6orf144 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Additional Text
Gene ID
389400
Gene Name
GFRAL
Uniprot ID
Q6UXV0
Protein Tag
hFC Tag
Short Description
Recombinant Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser19-Glu351.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
GFL-HM201 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations