Biotinylated Human GPRC5D Protein-VLP (GPR-HM05PB)
Catalogue Number: GPR-HM05PB-KAT
GPR-HM05PB Image
GPR-HM05PB Image
GPR-HM05PB Image
GPR-HM05PB Image
The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC.
GPR-HM05PB Image
Biotinylated Human GPRC5D VLP captured on SA Chip can bind Anti-GPRC5D antibody, hFc Tag with an affinity constant of 0.30 nM as determined in SPR assay (Biacore T200) (QC Test).
GPR-HM05PB Image
Immobilized Human CD3E&CD3D, hFc Tag at 1µg/ml (100µl/well) on the plate. Add serial dilutions of Anti-CD3xGPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The target protein has a predicted MW of 39.6 kDa. |
| Physical state: | Liquid |
| Type: | Protein - Conjugated |
| Alias: | GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor |
| Host Cell: | HEK293 cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ul |
| Application: | ELISA |
Description
Description: Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein-coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
Additional Text
Uniprot ID
Q9NZD1-1
Short Description
Recombinant Biotinylated Human GPRC5D Protein-VLP is expressed from HEK293.It contains Met1-Val345.
Storage Note
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
GPR-HM05PB Datasheet
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