Biotinylated Human GPRC5D Protein-Nanodisc (GPR-HM45PB)
Catalogue Number: GPR-HM45PB-KAT
GPR-HM45PB Image
GPR-HM45PB Image
GPR-HM45PB Image
GPR-HM45PB Image
GPR-HM45PB Image
Biotinylated Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 6.30 nM as determined in SPR assay (Biacore T200).
GPR-HM45PB Image
Immobilized Anti-GPRC5D Antibody, hFc Tag at 2µg/ml (100µl/well) on the plate. Dose response curve for Biotinylated Human GPRC5D Nanodisc, His Tag with the EC50 of 0.12µg/ml determined by ELISA (QC Test).
GPR-HM45PB Image
Immobilized Human CD3E&CD3D, His Tag at 2µg/ml (100µl/well) on the plate, add serial dilutions of Anti-Human CD3xGPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5µg/ml. Detection was performed using HRP-conjugated streptavidin with the EC50 of 0.28µg/ml determined by ELISA.
GPR-HM45PB Image
Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay (Gator Prime).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 42.10 kDa. |
| Physical state: | Liquid |
| Type: | Transmembrane Proteins |
| Host Cell: | HEK293 cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA |
Description
Description: Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein-coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
Additional Text
Protein Tag
HIS-AviTag™
Uniprot ID
Q9NZD1-1
Storage Note
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Short Description
Recombinant Biotinylated Human GPRC5D Protein-Nanodisc is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met1-Val345.
GPR-HM45PB Datasheet
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