Lamin A + C antibody [GT1137]
Catalogue Number: GTX00774-GTX
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ICC/IF analysis of H9C2 cells using GTX00774 Lamin A + C antibody [GT1137]. Green: Primary antibody Blue: DAPI Dlution : 1:200
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ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137]. Green: Primary antibody Blue: DAPI Dlution : 1:200
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ICC/IF analysis of L929 cells using GTX00774 Lamin A + C antibody [GT1137]. Green: Primary antibody Blue: DAPI The image was taken by Confocal microscopy. Dlution : 1:200
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ICC/IF analysis of HeLa cells using GTX00774 Lamin A + C antibody [GT1137]. Red: Primary antibody Blue: DAPI Detection system: confocol Dlution : 1:100
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ICC/IF analysis of U2OS cells using GTX00774 Lamin A + C antibody [GT1137]. Pink: Primary antibody Blue: DAPI Dlution : 1:100-1:1600
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IHC-P analysis of hmuan breast cancer tissue section using GTX00774 Lamin A + C antibody [GT1137]. Dlution : 1:200
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IHC-P analysis of hmuan esophageal cancer tissue section using GTX00774 Lamin A + C antibody [GT1137]. Dlution : 1:400 - 1:3200
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IP analysis of K562 whole cell lysate using GTX00774 Lamin A + C antibody [GT1137]. Total protein amount : 200µg IP antibody : 3µg Dilution : 1:20000
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WB analysis of HeLa whole cell lysate using GTX00774 Lamin A + C antibody [GT1137]. Dilution :1:10000-1:2560000 Loading : 25µg
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WB analysis of various samples using GTX00774 Lamin A + C antibody [GT1137]. Dilution : 1:50000 Loading : 25µg
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WB analysis of HeLa extracts using GTX00774 Lamin A + C antibody [GT1137]. WCE : whole cell lysate CE : cytoplasmic extracts NE : nuclear extracts Dilution : 1:10000 Loading : 25µg
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WB analysis of NIH-3T3 extracts using GTX00774 Lamin A + C antibody [GT1137]. WCE : whole cell lysate CE : cytoplasmic extracts NE : nuclear extracts Dilution : 1:10000 Loading : 25µg
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WB analysis of C6 extracts using GTX00774 Lamin A + C antibody [GT1137]. WCE : whole cell lysate CE : cytoplasmic extracts NE : nuclear extracts Dilution : 1:10000 Loading : 25µg
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WB analysis of Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts using GTX00774 Lamin A + C antibody [GT1137]. Dilution : 1:50000 Loading : 25µg
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT1137] (GTX00774) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide |
| Physical state: | Liquid |
| Type: | Recombinant Monoclonal |
| Alias: | lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | GT1137 |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of hmuan Lamin A/C |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Additional Text
Gene ID
4000
Uniprot ID
P02545
Purification
Affinity Purified
Molecular Weight
74
Gene Name
LMNA
Antibody Clonality
Recombinant Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:50000 - 1:200000. ICC/IF: 1:50 - 1:1000. IHC-P: 1:50 - 1:1000. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX00774 Datasheet
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