Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]
Catalogue Number: GTX00863-GTX
GTX00863 Image
GTX00863 Image
GTX00863 Image
GTX00863 Image
WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2. Lane 1 : Culture medium of E. coli O157:H7 Lane 2 : Purified VT1 Lane 3 : Purified VT2 Lane 4 : VT non-producing E. coli
GTX00863 Image
ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Protein coating amount : 100µl (1 µg/ml) per well Antibody amount : 100µl at indicated dilution
GTX00863 Image
Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | vero-01 |
| Isotype: | IgG1 |
| Immunogen: | Culture supernatant of E. coli 0111 ( EHEC strain) |
| Application: | ELISA, WB, LF |
Additional Text
Concentration
1 mg/ml
Purification
Protein A purified
Gene ID
1261950
Gene Name
stxA2
Antibody Clonality
Monoclonal
UniProt ID
P08026, P09385, Q9FBI2
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX00863 Datasheet
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