E-Cadherin antibody
Catalogue Number: GTX100443-GTX
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Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).
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E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. E-cadherin antibody (GTX100443) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 3 µg of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 µg E-cadherin antibody (GTX100443) 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 µg of E-Cadherin antibody (GTX100443). Western blot analysis was performed using E-Cadherin antibody (GTX100443). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody (GTX100443) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog skin. E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
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Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 µm.
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E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100443 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100443 Image
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-WM, PLA |
Description
Description: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
Additional Text
Gene Name
CDH1
Gene ID
999
Uniprot ID
P12830
Purification
Affinity Purified
Concentration
0.14 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
97
Application Notes
WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX100443 Datasheet
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