Vimentin antibody
Catalogue Number: GTX100619-GTX
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Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 µg of Vimentin antibody (GTX100619). Western blot analysis was performed using Vimentin antibody (GTX100619). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Vimentin antibody detects Vimentin protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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Vimentin antibody detects Vimentin protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract 10% SDS-PAGE Vimentin antibody (GTX100619) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (GTX100619) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:20000.
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Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat ovary by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Vimentin antibody detects Vimentin protein by immunohistochemical analysis. Sample: Paraffin-embedded dog pancreas. Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX100619 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody (GTX100619) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 µg/mL and GTX100619 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 µg/mL and GTX100619 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
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Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX100619 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100619 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX100619 Image
Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15
GTX100619 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100619 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100619 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX100619 Image
Vimentin antibody detects Vimentin protein at cytoskeleton and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
GTX100619 Image
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | vimentin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, IP, sELISA, WB, IHC-Fr, IHC, IHC-Flt |
Description
Description: Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Additional Text
Gene Name
VIM
Gene ID
7431
Uniprot ID
P08670
Purification
Affinity Purified
Molecular Weight
54
Concentration
1.43 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial-mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX100619 Datasheet
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