NFkB p65 antibody
Catalogue Number: GTX102090-GTX
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX102090) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NFkB p65 antibody (GTX102090) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000.
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Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102090 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102090 Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NFkB p65 stained by NFkB p65 antibody (GTX102090) diluted at 1:165. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | ChIP Validated Antibodies |
| Alias: | RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr |
Description
Description: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Additional Text
Gene Name
RELA
Gene ID
5970
Uniprot ID
Q04206
Concentration
0.49 mg/ml
Purification
Affinity Purified
Molecular Weight
60
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX102090 Datasheet
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