POM121 antibody [N2N3]
Catalogue Number: GTX102128-GTX
GTX102128 Image
GTX102128 Image
GTX102128 Image
GTX102128 Image
GTX102128 Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121(GTX102128) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11305 (Red) at 1:500.
GTX102128 Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121(GTX102128) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX102128 Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121(GTX102128) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX102128 Image
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] (GTX102128) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | POM121 transmembrane nucleoporin , P145 , POM121A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]
Additional Text
Gene Name
POM121
Gene ID
9883
Uniprot ID
Q96HA1
Concentration
0.16 mg/ml
Purification
Affinity Purified
Molecular Weight
128
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX102128 Datasheet
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